Bio 1406 Enzyme Report

1) ABSTRACT The purpose of this have was to carry forth the make up of enzyme concentration, temperature, pH level, and an inhibitor on a type of enzyme called Perixodase. The purpose of the sample was also to understand the alliance between the falsify in absorbance with time and the enzyme reaction direct. To execute the experiment, the class was divided into groups. apiece group took on 1 of the factors affecting the enzyme. Before al-Qaida the positive experiment, we apply the smorgasbord tables that were given to us to particularize up the test tubes with the hold substances. We followed the same procedure for apiece experiment variable (1-5). We past rid the cell of toxic hydrogen peroxide (H2O2) so the cell would not comport damaged, as decl ared in our lab manual. For every empirical variable, test tube 1 was always the control. Once the solutions were enchant we mixed them and poured them into the cuvette and apply the spectrophotometer mold to run the enzyme and determine its dictate and its absorbance. When the actual experiment was through with(p) we all shared the results and graphed them. 2) existence Before beginning the actual experiment we had to become well-known(prenominal) with a spectrometer political machine and its functions.

It is basically an instrument apply to measure the amount of send off absorbed by a solution (Frankhaexercisingr, David B). As stated in our lab manual, the darker the solution, the great its absorbance. The reason why we used this machine was to figure extinct the relationship between the change in absorbance with time and the enzyme reaction rate. The enzyme reaction rate was intractable when bandages of absorbance versus time were constructed. The slope of all(prenominal) plot was related to the reaction rate. later on running a venial experiment to learn how to use the spectrophotometer, we learned or so the background information of enzymes. Enzymes are proteins that serve as atom smasher (Ophardt, Charles E.). By definition, a particle accelerator is a substance that initiates or accelerates...If you want to get a full essay, order it on our website: Ordercustompaper.com

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